ELISA Test – ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In an ELISA, an antigen must be immobilized to a solid surface and Baca selengkapnya
Sandwich ELISA - All of the ELISA kits from Boster use the sandwich format and avidin-biotin chemistry. Our ELISA Baca selengkapnya
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Competitive ELISA : Antigen Coating, Blocking, Preparation
Competitive ELISA, This is a general protocol in which antigen coating and blocking may not be required if the wells from the manufacturer have been pre-adsorbed with the antigen. 1. Antigen Coating Dilute purified antigens Baca selengkapnya
Sandwich ELISA – Antibody Coating, Blocking, Preparation
Sandwich ELISA – All of the ELISA kits from Boster use the sandwich format and avidin-biotin chemistry. Our ELISA assays require the dilutions of standard solutions, biotinylated antibody (detection antibody) and avidin-biotinperoxidase. 1. Capture Antibody Baca selengkapnya
Indirect ELISA – Antigen Coating, Blocking, Preparation
Indirect ELISA – This is a general protocol in which antigen coating and blocking may not be required if the wells from the manufacturer have been pre-adsorbed with the antigen. 1. Antigen Coating Dilute purified Baca selengkapnya
Illumina/Solexa Sequencing
In the mid-1990s, scientists at Cambridge, Shankar Balasubramanian and David Klenerman, developed methods to produce high-quality reads of much greater data size at a reduced cost. In this method single DNA molecules are attached to Baca selengkapnya
Roche/454 Sequencing
Roche/454 sequencing is the pioneer NGS platform and was launched in 2005 by Rothberg and colleagues and is popularly referred to as pyrosequencing (Margulies et al. 2005). The technique is based on sequencing-by-synthesis approach and uses Baca selengkapnya
HIV Genotype and Phenotype Testing
Development of drug resistance to antiretroviral therapy HIV Genotype and Phenotype Testing- Human immunodeficiency virus (HIV) replicates rapidly, particularly in response to antiretroviral (ARV) therapies. These replications are a result of substitutions in the viral Baca selengkapnya
Principles Of Insurance
Let’s to understand about principles of insurance! 1. Law of large numbers Imagine that in a village there are 1000 persons who are all aged 50 and are healthy. Based on previous experience, it is Baca selengkapnya
What is insurance Policy?
Insurance is a way to manage your risk. When you buy insurance, you purchase protection against unexpected financial losses. The insurance company pays you or someone you choose if something bad happens to you. If Baca selengkapnya
