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In the mid-1990s, scientists at Cambridge, Shankar Balasubramanian and David Klenerman, developed methods to produce high-quality reads of much greater data size at a reduced cost. In this method single DNA molecules are attached to

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Roche/454 sequencing is the pioneer NGS platform and was launched in 2005 by Rothberg and colleagues and is popularly referred to as pyrosequencing (Margulies et al. 2005). The technique is based on sequencing-by-synthesis approach and uses

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Development of drug resistance to antiretroviral therapy HIV Genotype and Phenotype Testing- Human immunodeficiency virus (HIV) replicates rapidly, particularly in response to antiretroviral (ARV) therapies. These replications are a result of substitutions in the viral